In Vitro somatic embryogenesis in Hardwickia binata. (a) Development of embryogenic callus from immature zygotic embryos on MS medium + 1.0 mg/l Kn, 2.0 mg/l 2,4-D after 4 weeks of culture. (b, c) Secondary somatic embryogenesis on medium having 1.0 mg/l Kn, 2.0 mg/l 2,4-D after 4 weeks of subculture. (d) Globular and torpedo shaped somatic embryos germinating on strength MS medium supplemented with 0.1 mg/l IAA, 0.25 mg/l BA and 2% sucrose after 2 weeks of culture.
