Table of Contents
Biotechnology Research International
Volume 2011, Article ID 810425, 8 pages
http://dx.doi.org/10.4061/2011/810425
Research Article

Optimization for the Production of Cellulase Enzyme from Municipal Solid Waste Residue by Two Novel Cellulolytic Fungi

1Central Pollution Control Board, New Delhi, India
2Regional Office, M. P. Pollution Control Board, Vijay Nagar, Jabalpur, Madhya Pradesh, India
3Mycological Research Laboratory, Department of Biological Sciences, Rani Durgavati University, Jabalpur, Madhya Pradesh, India
4Yeast and Mycorrhiza Biotechnology Laboratory, Department of Biological Sciences, Rani Durgavati University, Jabalpur, Madhya Pradesh, India

Received 13 November 2010; Accepted 24 December 2010

Academic Editor: Triantafyllos Roukas

Copyright © 2011 S. P. Gautam et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus niger and Trichoderma sp.) from municipal solid waste. Municipal solid waste residue (4-5% (w/v)) and peptone and yeast extract (1.0% (w/v)) were found to be the best combination of carbon and nitrogen sources for the production of cellulase by A. niger and Trichoderma sp. Optimum temperature and pH of the medium for the cellulase production by A. niger were 40°C and 6-7, whereas those for the production of cellulase by Trichoderma sp. were 45°C and 6.5. Cellulase production from A. niger and Trichoderma sp. can be an advantage as the enzyme production rate is normally higher as compared to other fungi.