Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces
Figure 2
Two-dimensional separation of hCASMC lysates harvested from uncoated glass (top), (PDADMA/PSS)2 (bottom left), and (PFPVP/Nafion)2 (bottom right). Representative gels show the separation of 50 μg of total protein separated from pH 4 to 7 on a 10% polyacrylamide gel stained with SYPRO Ruby and imaged with Typhoon Scanning Systems. White arrows correspond to excised spots that were identified as HSP90 (top-left; MW: 85 kDa, pI: 4.9, with α and β isoforms sharing these approximate values), actin (bottom-right; MW 42 kDa, pI: 5.2, with the β, γ, and α-SM isoforms sharing these approximate values), and vimentin (center; MW: 53 kDa, pI: 5.1).
