Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces
Figure 7
Fluorescent staining for vimentin on (a) uncoated glass, (b) (PFPVP/Nafion)2, (c, e) (PDADMA/PSS)2, and (d, f) (PAH/PAA-co-PAEDAPS)2. The top four images (a)–(d) are confocal microscopy images in which positive signals are detected with the Alexa Fluor 546 secondary antibody conjugated to a red fluorophore, and cell nuclei are stained blue with DAPI. The lower images (e, f) are wide-field images at the same magnification for enhanced visualization of (e) the fine mesh of intermediate filaments on the (PDADMA/PSS)2 surface and (f) formation of the condensed “nuclear cap” on the (PAH/PAA-co-PAEDAPS)2 surface.
