Human Coronary Artery Smooth Muscle Cell Responses to Bioactive Polyelectrolyte Multilayer Interfaces
Figure 8
Contraction of hCASMC culture on a (PFPVP/Nafion)2 surface. Images are shown at (a) 0 minutes, (b) 2.5 minutes, (c) 3.5 minutes, and (d) 5 minutes after the initiation of contraction using the A23187 ionophore. A contractile “hill and valley” morphology (a) is illustrated by the thick arrows (“hills”) and the thin arrows (“valleys”). This response was not observed on glass or (PDADMA/PSS)2 cultures exposed to the A23187 ionophore, nor was it seen in control (PFPVP/Nafion)2 cultures exposed to SmBM/DMSO alone, which served as the vehicle for A23187 delivery (data not shown). (PAH/PAA-co-PAEDAPS)2 cultures were not tested by these methods. Representative image stills are from an image stack of 100 images, all taken at 100x and processed using Metamorph Systems. Scale bar = 20 μm.
