Generation of HIV-1 and Internal Control Transcripts as Standards for an In-House Quantitative Competitive RT-PCR Assay to Determine HIV-1 Viral Load

Table 1

Sequences of the primers and probes used in this study.

Code	Polarity^a	Primer or probe sequence (5′ to 3′)	Source
Primer HIV1-Q3	+	CTCAATAAAGCTTGCCTTGA	[15]
Primer HIV1-Biot-Q4	−	GGGCGCCACTGCTAGAGA	[15]
Primer IC-1	+	CTCAATAAAGCTTGCCTTGATAGTTGAATTGAAAGTGC	This study
Primer IC-2	−	GGGCGCCACTGCTAGAGAATAGTGTCGCCCACGTTTAC	This study
T7 promoter primer	+	TAATACGACTCACTATAGGG	[16]
Probe HIV1-S	None	AGTAGTGTGTGCCCGTCTGT	[15]
Probe IC-S1	None	TAGTTGAATTGAAAGTGCCCGACAT	This study

^aPolarity: (+) sense, (−) antisense.