Generation of HIV-1 and Internal Control Transcripts as Standards for an In-House Quantitative Competitive RT-PCR Assay to Determine HIV-1 Viral Load

Table 5

Determination of the HIV-1 transcript (T-HIV1-2^b) RNA concentration in a qRT-PCR assay.

Name	T-HIV1-2^b dilution	HIV-1 RNA (IU/mL)	Mean of the HIV-1 RNA (IU/mL)
T-HIV1-2^b	1 : 4 × 10^7	Non quantifiable	1.27E  + 14
	1 : 4 × 10^8	8.83𝐸+13
	1 : 4 × 10^9	1.29𝐸+14
	1 : 4 × 10^9	1.50𝐸+14
	1 : 4 × 10^10	1.02𝐸+14
	1 : 4 × 10^10	7.05𝐸+13
	1 : 4 × 10^11	2.21𝐸+14