Generation of HIV-1 and Internal Control Transcripts as Standards for an In-House Quantitative Competitive RT-PCR Assay to Determine HIV-1 Viral Load

Table 7

Determination of the lower limit detection (LoD) of the in-house qRT-PCR assay.

HIV-1 IS RNA concentrations^a (IU/mL)	Number of replicas tested	Positive results	Positive rate (%)
5000	6	6	100
2500	6	6	100
1000	11	11	100
500	15	14	93.3
250	13	3	23.1
100	11	1	9.1
50	5	0	0

^aThe HIV-1 IS RNA concentrations were obtained from dilutions made to the HIV-1 international standard (code 97/656) that contained 100 000 IU/mL (reconstituted in 1 mL of ultrapure water) as instructed by the NIBSC manual.