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Biotechnology Research International
Volume 2012 (2012), Article ID 934325, 7 pages
Research Article

Production of Alkaline Cellulase by Fungi Isolated from an Undisturbed Rain Forest of Peru

Laboratorio de Micología y Biotecnología, Universidad Nacional Agraria La Molina, Avenida La Molina s/n, Lima 12, Peru

Received 14 September 2012; Accepted 20 October 2012

Academic Editor: Triantafyllos Roukas

Copyright © 2012 Karin Vega et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Alkaline cellulase producing fungi were isolated from soils of an undisturbed rain forest of Peru. The soil dilution plate method was used for the enumeration and isolation of fast growing cellulolytic fungi on an enriched selective medium. Eleven out of 50 different morphological colonies were finally selected by using the plate clearing assay with CMC as substrate at different pH values. All 11 strains produced cellulases in liquid culture with activities at alkaline pH values without an apparent decrease of them indicating that they are true alkaline cellulase producers. Aspergillus sp. LM-HP32, Penicillium sp. LM-HP33, and Penicillium sp. LM-HP37 were the best producers of FP cellulase (>3 U mL−1) with higher specific productivities (>30 U g−1 h−1). Three strains have been found suitable for developing processes for alkaline cellulase production. Soils from Amazonian rain forests are good sources of industrial fungi with particular characteristics. The results of the present study are of commercial and biological interest. Alkaline cellulases may be used in the polishing and washing of denim processing of the textile industry.