Table of Contents
Biotechnology Research International
Volume 2013 (2013), Article ID 137851, 5 pages
Research Article

Statistical Analysis of Metal Chelating Activity of Centella asiatica and Erythroxylum cuneatum Using Response Surface Methodology

1Natural Product Division, Drug Discovery Centre (DDC), Forest Research Institute Malaysia (FRIM), Jalan Kepong, Selangor Darul Ehsan, 52109 Kepong, Malaysia
2Malaysian Institute of Pharmaceuticals and Nutraceuticals, Ministry of Science, Technology and Innovation, USM, 10 Persiaran Bukit Jambul, 11900 Bukit Jambul, Malaysia
3Department of Biotechnology Engineering, International Islamic University Malaysia, Gombak, P.O. Box 10, 50728 Kuala Lumpur, Malaysia

Received 24 October 2012; Accepted 20 December 2012

Academic Editor: Triantafyllos Roukas

Copyright © 2013 R. J. Mohd Salim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The purpose of the study is to evaluate the relationship between the extraction parameters and the metal chelating activity of Centella asiatica (CA) and Erythroxylum cuneatum (EC). The response surface methodology was used to optimize the extraction parameters of methanolic extract of CA and EC with respect to the metal chelating activity. For CA, Run 17 gave optimum chelating activity with IC50 = 0.93 mg/mL at an extraction temperature of 25°C, speed of agitation at 200 rpm, ratio of plant material to solvent at 1 g : 45 mL and extraction time at 1.5 hour. As for EC, Run 13 with 60°C, 200 rpm, 1 g : 35 mL and 1 hour had metal chelating activity at IC50 = 0.3817 mg/mL. Both optimized extracts were further partitioned using a solvent system to evaluate the fraction responsible for the chelating activity of the plants. The hexane fraction of CA showed potential activity with chelating activity at IC50 = 0.090 and the ethyl acetate fraction of EC had IC50 = 0.120 mg/mL. The study showed that the response surface methodology helped to reduce the extraction time, temperature and agitation and subsequently improve the chelating activity of the plants in comparison to the conventional method.