Statistical Analysis of Metal Chelating Activity of Centella asiatica and Erythroxylum cuneatum Using Response Surface Methodology

Table 3

Face centered, central composite design setting with the independent variables and their responses in EC.

Run number	𝑋1	𝑋2	𝑋3	𝑋4	Y; 1/IC50	IC50
1	60	200	35	60	2.4	0.4167
2	55	150	40	30	1.3	0.7692
3	55	250	30	90	1.4	0.7143
4	60	150	35	60	2.1	0.4762
5	60	200	30	60	1.5	0.6667
6	65	150	30	90	0.76	1.3158
7	65	150	30	30	1.18	0.8475
8	60	200	40	60	1.59	0.6289
9	60	200	35	60	2.6	0.3846
10	55	250	30	30	0.909	1.1001
11	65	150	40	30	1.1	0.9091
12	55	150	30	90	1.59	0.6289
13	60	200	35	60	2.62	0.3817
14	65	150	40	90	0.7	1.4286
15	60	200	35	60	2.57	0.3891
16	55	250	40	90	1.39	0.7194
17	60	200	35	90	1.57	0.6369
18	65	200	35	60	1.68	0.5952
19	60	200	35	60	2.56	0.3906
20	55	200	35	60	1.89	0.5291
21	60	200	35	30	1.55	0.6452
22	60	250	35	60	2.1	0.4762
23	65	250	30	90	0.833	1.2005
24	65	250	30	30	1.25	0.8000
25	60	200	35	60	2.56	0.3906
26	55	150	30	30	1.28	0.7813
27	65	250	40	30	1.36	0.7353
28	55	150	40	90	1.66	0.6024
29	65	250	40	90	0.906	1.1038
30	55	250	40	30	1.1	0.9091