Family-Specific Degenerate Primer Design: A Tool to Design Consensus Degenerated Oligonucleotides
Minimum degenerated sequence generation. (a) Diagram of the general strategy used. (b) Sample protein alignment showing an example for the steps of the strategy diagram. Each sequence is computationally backtranslated to hypothetical nucleic acid sequences. IUPAC codes were used to show ambiguous positions. These sequences are piled up in order to get the degenerated consensus sequence. Numbers below this indicate the degeneration value of each position.
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