Family-Specific Degenerate Primer Design: A Tool to Design Consensus Degenerated Oligonucleotides
Experimental challenge of designed primers. (a) Genomic organization of the Arenaviruses S RNA and P74 ORF. Arenavirus shows an ambisense coding strategy of the GPC and N ORFs and three noncoding regions: 5′ untranslated region (5UTR), intergenic region (IGR), and 3′ untranslated region (3UTR). The location of each designed primer (GR1058, N918, N537, and p74-1334r) and specific primers (Arena, p74-550) is also shown. (b) The results obtained with each pair of primers tested and characteristics of reaction are shown.
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