Table of Contents
Biotechnology Research International
Volume 2013, Article ID 435154, 7 pages
Research Article

Production of Pectinolytic Enzymes by the Yeast Wickerhanomyces anomalus Isolated from Citrus Fruits Peels

1Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Felix de Azara 1552, N3300LQH Posadas, Argentina
2Universidad Nacional del Chaco Austral, Comandante Fernández 755, H3700LGO Presidencia Roque Sáenz Peña, Argentina
3Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI, UNLP, CONICET La Plata), Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Calle 47 y 115, B1900ASH La Plata, Argentina

Received 26 November 2012; Accepted 7 February 2013

Academic Editor: Triantafyllos Roukas

Copyright © 2013 María A. Martos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Wickerhamomyces anomalus is pectinolytic yeast isolated from citrus fruits peels in the province of Misiones, Argentine. In the present work, enzymes produced by this yeast strain were characterized, and polygalacturonase physicochemical properties were determined in order to evaluate the application of the supernatant in the maceration of potato tissues. W. anomalus was able to produce PG in liquid medium containing glucose and citrus pectin, whose mode of action was mainly of endo type. The supernatant did not exhibit esterase or lyase activity. No others enzymes, capable of hydrolyzing cell wall polymers, such as cellulases and xylanases, were detected. PG showed maximal activity at pH 4.5 and at temperature range between 40°C and 50°C. It was stable in the pH range from 3.0 to 6.0 and up to 50°C at optimum pH. The enzymatic extract macerated potato tissues efficiently. Volume of single cells increased with the agitation speed. The results observed make the enzymatic extract produced by W. anomalus appropriate for future application in food industry, mainly for the production of fruit nectars or mashed of vegetables such as potato or cassava, of regional interest in the province of Misiones, Argentine.