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Biotechnology Research International
Volume 2014 (2014), Article ID 109249, 6 pages
Research Article

Biotransformation of Indigo Pigment by Indigenously Isolated Pseudomonas sp. HAV-1 and Assessment of Its Antioxidant Property

1Department of Microbiology, P. D. Patel Institute of Applied Sciences, Charotar University of Science & Technology, Changa, Gujarat 388 421, India
2Department of Biotechnology, Ashok & Rita Patel Institute of Integrated Studies and Research in Biotechnology and Allied Sciences (ARIBAS), Sardar Patel University, New Vallabh Vidyanagar, Gujarat 388 121, India

Received 13 August 2014; Revised 8 October 2014; Accepted 22 October 2014; Published 17 November 2014

Academic Editor: Yau Hung Chen

Copyright © 2014 Aditi Dua et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Chemical synthesis of indigo poses harsh environmental hazards and adverse human health effects. This necessitates an environment-friendly and producer-friendly approach for indigo production. The present study was thus significant as it reports an indigenously isolated potential indigo pigment producing culture identified as Pseudomonas sp. HAV-1 with noteworthy antioxidant property. The bioindigo pigment was characterized using various analytical techniques. The pigment production was enhanced from 412 μg mL−1 to 700 μg mL−1 by optimizing the growth parameters. Furthermore, the antioxidant property of indigo pigment is hitherto unexplored. This property can significantly append to its therapeutic potential. The bioindigo pigment produced by Pseudomonas sp. HAV-1 depicted 2.2 μM ascorbic acid equivalent antioxidant property. More to the point, the present work addresses a footstep towards green production of indigo.