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Biotechnology Research International
Volume 2014 (2014), Article ID 217030, 8 pages
http://dx.doi.org/10.1155/2014/217030
Research Article

Production of Bioactive Compounds by Actinomycetes and Their Antioxidant Properties

Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh 517502, India

Received 17 December 2013; Accepted 20 February 2014; Published 26 March 2014

Academic Editor: Triantafyllos Roukas

Copyright © 2014 Avilala Janardhan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An actinomycete was isolated from mangrove soil collected from Nellore region of Andhra Pradesh, India, and screened for its ability to produce bioactive compounds. The cultural, morphological, and biochemical characters and 16S rRNA sequencing suggest that the isolated strain is Nocardiopsis alba. The bioactive compounds produced by this strain were purified by column chromatography. The in vitro antioxidant capacity of the isolated compounds (fractions) was estimated and fraction F2 showed very near values to the standard ascorbic acid. The potential fraction obtained by column chromatography was subjected to HPLC for further purification, then this purified fraction F2 was examined by FTIR, NMR, and mass spectroscopy to elucidate its chemical structure. By spectral data, the structure of the isolated compound was predicted as “(Z)-1-((1-hydroxypenta-2,4-dien-1-yl)oxy)anthracene-9,10-dione.”