In Vitro Flower Induction from Shoots Regenerated from Cultured Axillary Buds of Endangered Medicinal Herb Swertia chirayita H. Karst.
Table 1
S. number
Photoperiod (light/dark)
Carbohydrate source
Subculture duration
S
G
F
M
L
4 wks
6 wks
8 wks
1
12 h/12 h
b
c
c
c
d
d
c
c
2
16 h/8 h
a
b
b
b
d
d
a
b
3
8 h/16 h
d
d
d
d
d
d
d
d
S: sucrose, G: glucose, F: fructose, M: maltose, and L: lactose (All 2.5%). Flowering response: a: average of 12 flower buds per culture, b: average of 6 flower buds per culture, c: average of 4 flower buds per culture, and d: no flowering. All cultures were maintained on full strength MS medium with 1 mg/L BAP and .007% adenine sulfate.
