Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3

Table 1

(a) Plackett-Burman experimental design for screening of important chemical determinants of protease production by X. indica KB-3. (b) Twelve-trial Plackett-Burman design matrix for chemical components and their respective observed protease activity.

(a)

Variable	Unit	Code	Experimental value
			Lower (−1)	Center (0)	Higher (+1)
Tryptone	g/L	𝐴	3.0	5.0	7.0
Soya peptone	g/L	𝐵	4.5	5.0	5.5
Dextrose	g/L	𝐶	1.0	1.5	2.0
NaCl	g/L	𝐷	5.0	6.0	7.0
Dipotassium phosphate	g/L	𝐸	0.75	1.0	1.25

(b)

Standard order	Experimental values					Protease production; (U/mL)
	𝐴	𝐵	𝐶	𝐷	𝐸
1	7	5.5	1	7	1.25	1164.83±0.21
2	3	5.5	2	5	1.25	1018.50±0.13
3	7	4.5	2	7	0.75	1305.50±0.17
4	3	5.5	1	7	1.25	1129.17±0.09
5	3	4.5	2	5	1.25	878.00±0.25
6	3	4.5	1	7	0.75	1065.83±0.32
7	7	4.5	1	5	1.25	709.50±0.08
8	7	5.5	1	5	0.75	932.50±0.02
9	7	5.5	2	5	0.75	1134.50±0.22
10	3	5.5	2	7	0.75	1409.50±0.17
11	7	4.5	2	7	1.25	1231.17±0.13
12	3	4.5	1	5	0.75	748.167±0.07