Characterization of β-Glucosidase Produced by Aspergillus niger under Solid-State Fermentation and Partially Purified Using MANAE-Agarose

Table 2

Mass balance of β-glucosidase purification using MANAE-agarose support in a chromatographic column.

	MANAE-agarose
	Total protein		β-Glucosidase
	mg	%	IU	%
Feeding	2.95	100	33.08	100
Washed out	1.96	66.4	25.08	75.8
Elution NaCl (mM)
50	0.07	2.4	0.03	0.1
150	0.39	13.2	0.14	0.4
300	0.41	13.9	7.00	21.2
600	0.07	2.4	0.20	0.6
Total recovery	2.90	98.3	32.5	98.2