Overcome of Carbon Catabolite Repression of Bioinsecticides Production by Sporeless Bacillus thuringiensis through Adequate Fermentation Technology

Table 2

Aeration effect on delta-endotoxin production in glucose based medium.

Aeration profiles; (% oxygen saturation)	Total production (mg)	CFU; (10^7 cells/mL)	Toxin synthesis yield (mg/10^10 cells)**	Toxin production yield (mg/g glucose)***	LC50 (ng of toxin per mg of flour)
60–40		18 ± 4.3^a	120.5	113.41	560.86 ± 8.59^bc
70–40	3704 ± 90.5^b	15 ± 3^a	120.46	96.83	574.65 ± 13.06^b
60–30	4202 ± 146.6^a	20 ± 3.6^a	105.05	109.85	546.61 ± 15.16^c
40-40	3064 ± 26.5^d	17 ± 2.6^a	97.41	80.1	604.09 ± 7.09^a
60–50	3430 ± 98.2^c	15 ± 2.6^a	114.33	89.67	592.54 ± 3.29^a

; coefficient of variance = 2.84; (Pr > ) < 0.001.
*The letters a, b, c, and d used as indices in the same column indicated the presence of significant differences (Duncan’s multiple range test, < 0.05) among the experiments, in ascending order.
**Relative toxin yield calculated as the ratio of delta-endotoxins (mg/L) over CFU (10¹⁰ cells/L) both of them determined at the end of fermentation.
***Toxin production yield calculated as the ratio of delta-endotoxin (mg/L) over assimilated glucose (g/L).