Overcome of Carbon Catabolite Repression of Bioinsecticides Production by Sporeless Bacillus thuringiensis through Adequate Fermentation Technology

Table 3

Aeration effect on delta-endotoxins production in media based on different concentrations of glucose and yeast extract.

Aeration profiles	Glucose (mg/L)	Yeast extract (g/L)	Total production (mg)	CFU (10^7 cells/mL)	Toxin synthesis yield (mg/10^10 cells)	Toxin production yield (mg/g glucose)	LC50 (ng of toxin per mg of flour)
60–40	30	5.8		22 ± 2.82^f	107.08	78.52	604.8 ± 2.99^a
75-75	30	5.8	4871 ± 148.49^b	39 ± 5.65^c,d	73.46	95.5	574 ± 5.65^b,c
60–40	40	5.8	4107 ± 111.72^c,d	26 ± 5.65^e,f	92.91	60.39	592.11 ± 24.52^a,b
75-75	40	5.8	4871.5 ± 149.19^b	44 ± 0^b,c,d	65.12	71.63	557.88 ± 5.35^c
60–40	30	10.31	4382.5 ± 38.89^c	35 ± 1.41^d,e	73.65	85.93	579.44 ± 4.76^a,b,c
75-75	30	10.31	4966 ± 106^a,b	48 ± 2.82^b,c	60.85	97.37	554.72 ± 0.88^c
60–40	40	10.31	4356 ± 31.11^c	51 ± 7.07^b	50.24	64.05	580 ± 11.31^a,b,c
75-75	40	10.31	5195 ± 145.66^a	66 ± 5.65^a	46.3	76.39	556.39 ± 7.46^c

The letters a, b, c, d, e, and f used as indices in the same column indicated the presence of significant differences (Duncan’s multiple range test, < 0.05) among the experiments, in ascending order.