Overcome of Carbon Catabolite Repression of Bioinsecticides Production by Sporeless Bacillus thuringiensis through Adequate Fermentation Technology

Table 5

Effect of glucose feeding on production of bioinsecticides into 3 litre fermenter.

Initial concentration of glucose (g/L)	Total glucose consumption (g/L)	Yeast extract (g/L)	Total production (mg)	CFU (10^7 cells/mL)	Toxin synthesis yield (mg/10^10 cells)	Toxin production yield (mg/g glucose)	LC50 (ng of toxin per mg of flour)
22.5**	22.5	5.8		22 ± 4.5^a	94.54	109.85	626.87 ± 24.03^a
22.5	35	5.8	5712 ± 141.7^b	25 ± 7.9^a	95.2	96	553.35 ± 11.91^c
22.5***	34.07	10	5650 ± 70.3^b	26 ± 8.1^a	94.46	97.55	542.32 ± 9.26^c
10	18.77	5.8	3126 ± 16^e	15 ± 5.2^a	94.66	97.96	561.41 ± 5.69^c
15	29	5.8	4831 ± 150^c	22 ± 9.1^a	99.81	97.99	556.29 ± 14.37^c
30	30	5.8	4035 ± 106.8^d	22 ± 11.5^a	83	79.11	620.37 ± 18.45^a
40	40	5.8	4107 ± 81^d	30 ± 6^a	62.23	60.39	589.85 ± 17.81^b
22.5	42.5	5.8	6503 ± 49.1^a	28 ± 7.5^a	96.75	90	552.61 ± 11.55^c

= 0.994; coefficient of variance = 2.17; (Pr > ) < 0.001.
*The letters a, b, c, d, and e used as indices in the same column indicated the presence of significant differences (Duncan’s multiple range test, < 0.05) among the experiments, in ascending order.
**Batch culture curried out with the mentioned carbon source and yeast extract concentrations.
***Fed-batch culture realized with feeding both glucose and yeast extract.