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Biotechnology Research International
Volume 2014 (2014), Article ID 705068, 6 pages
Research Article

Characterization of Modified Magnetite Nanoparticles for Albumin Immobilization

1Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran
2Department of Biotechnology, Faculty of New Science and Technology, University of Isfahan, Isfahan 81746-73441, Iran
3Department of Molecular and Cell Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Received 5 February 2014; Accepted 28 April 2014; Published 22 May 2014

Academic Editor: Manuel Canovas

Copyright © 2014 A. K. Bordbar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Magnetite Fe3O4 nanoparticles (NPs) were prepared by chemical coprecipitation method. Silica-coated magnetite NPs were prepared by sol-gel reaction, subsequently coated with 3-aminopropyltriethoxysilane (APTES) via silanization reaction, and then were activated with 2,4,6-trichloro-1,3,5-triazine (TCT) and covalently immobilized with bovine serum albumin (BSA). The size and structure of the particles were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and dynamic light scattering (DLS) techniques. The immobilization was confirmed by Fourier transform infrared spectroscopy (FT-IR). XRD analysis showed that the binding process has not done any phase change to Fe3O4. The immobilization time for this process was 4 h and the amount of immobilized BSA for the initial value of 1.05 mg BSA was about 120 mg/gr nanoparticles. Also, the influences of three different buffer solutions and ionic strength on covalent immobilization were evaluated.