Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases

<table><tr><td><table class="algorithm" id="figbox1"><tr><td colspan="2">High affinity + high specificity<svg height="16.8375" id="M32" style="vertical-align:-10.00693pt" version="1.1" viewbox="0 0 22.012501 16.8375" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg>medium reversibility<svg height="16.8375" id="M33" style="vertical-align:-10.00693pt" version="1.1" viewbox="0 0 22.012501 16.8375" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg>medium</td></tr><tr><td colspan="2">toxicity </td></tr><tr><td colspan="2">high affinity + low specificity<svg height="16.8375" id="M34" style="vertical-align:-10.00693pt" version="1.1" viewbox="0 0 22.012501 16.8375" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg>low reversibility<svg height="16.8375" id="M35" style="vertical-align:-10.00693pt" version="1.1" viewbox="0 0 22.012501 16.8375" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg>high toxicity</td></tr><tr><td colspan="2">low affinity + medium specificity<svg height="16.8375" id="M36" style="vertical-align:-10.00693pt" version="1.1" viewbox="0 0 22.012501 16.8375" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg>medium reversibility<svg height="16.8375" id="M37" style="vertical-align:-10.00693pt" version="1.1" viewbox="0 0 22.012501 16.8375" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg>medium</td></tr><tr><td colspan="2">toxicity</td></tr></table></td></tr></table>

Biotechnology Research International

figbox1

Box 1

Box 1: Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases 