Research Article
In Silico Analysis of Bioethanol Overproduction by Genetically Modified Microorganisms in Coculture Fermentation
Table 2
Metabolic engineering strategies for ethanol overproduction by recombinant
E. coli strain ZSC113 [
22].
| Number | Metabolic engineering strategy | Deleted reaction |
| 1 | Deletion of acetate kinase (ack) | Acetate + ATP ↔ Acetyl phosphate + ADP |
| 2 | Deletion of pyruvate formate lyase (pfl) | Acetyl-CoA + Formate → CoA + Pyruvate |
| 3 | Deletion of phosphotransacetylase (pta) | Acetyl-CoA + Phosphate ↔ Acetyl phosphate + CoA |
| 4 | Deletion of fumarase (fum) + pfl | Fumarate + H2O ↔ L-malate Acetyl-CoA + Formate → CoA + Pyruvate |
| 5 | Deletion of phosphogluconate dehydrogenase (gnd) + ack | 6-Phospho-D-gluconate + NADP → Ribulose 5-phosphate + NADPH + CO2 Acetate + ATP ↔ Acetyl phosphate + ADP |
| 6 | Deletion of glutamate dehydrogenase (gdh) + pta | Glutamate + NADP + H2O ↔ 2-Oxoglutarate + NADPH + NH3 Acetyl-CoA + Phosphate ↔ Acetyl phosphate + CoA |
| 7 | gdh + ack | Glutamate + NADP + H2O ↔ 2-Oxoglutarate + NADPH + NH3 Acetate + ATP ↔ Acetyl phosphate + ADP |
| 8 | gdh + pfl | Glutamate + NADP + H2O ↔ 2-Oxoglutarate + NADPH + NH3 Acetyl-CoA + Formate → CoA + Pyruvate |
| 9 | Deletion of methylenetetrahydrofolate dehydrogenase (mthfd) + ∆ack | 5,10-Methylenetetrahydrofolate + NADP ↔ 5,10-Methenyltetrahydrofolate + NADPH Acetate + ATP ↔ Acetyl phosphate + ADP |
| 10 | Deletion of carbamate kinase (cbm) + ack | ATP + CO2 + NH4 ↔ ADP + Carbamoyl phosphate Acetate + ATP ↔ Acetyl phosphate + ADP |
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