In Silico Analysis of Bioethanol Overproduction by Genetically Modified Microorganisms in Coculture Fermentation

Table 3

Metabolic engineering strategies for ethanol overproduction by S.  cerevisiae [12].

Number	Metabolic engineering strategy	Inserted reaction
1	Insertion of NADP dependent glycerol 3-phosphate dehydrogenase (R00845)	Glycerol 3-phosphate + NADP ↔ D-Glyceraldehyde 3-phosphate + NADPH
2	Insertion of nonphosphorylating NADP dependent glyceraldehyde-3-phosphate dehydrogenase (R01058)	D-glyceraldehyde 3-phosphate + NADP → 3-phospho-D-glycerate + NADPH