Application of Two-Stage Cultivation for Exploring the Nutritional Requirements for Sporulation of Three Biocontrol Fungi

Table 1

Carbon concentrations and C/N ratios for sporulation.

Isolates	Host	Collection places	C/N ratio	Carbon concentration (g/L)	Carbon sources	Nitrogen sources	Minerals and vitamins
B. bassiana (IBC1201)	Locust	Tianjin, China	5 : 1	4	Sucrose, glucose, cellobiose	Yeast extract, urea, soy peptone	ZnSO4⋅7H2O 50 mg/L, CuSO4⋅5H2O 50 mg/L, CaCl2 g/L, MnSO4⋅H2O 5 mg/L
L. lecanii (CA-1-G)	Myzus persicae	Fujian, China	5 : 1	4	Glucose, trehalose, cellobiose	Yeast extract, soy peptone, urea	CuSO4⋅5H2O 10 mg/L, ZnSO4⋅7H2O 50 mg/L, CaCl2 1 g/L, H3BO4 5 mg/L, Na2MoO4⋅2H2O 5 mg/L
P. chlamydosporia (HSY-12-14)	Meloidogyne incognita	Hainan, China	10 : 1	8	Sucrose, maltose	NaNO3, urea	ZnSO4⋅7H2O 50 mg/L, H3BO4 50 mg/L, MnSO4⋅H2O 50 mg/L