Extracellular α-Galactosidase from Trichoderma sp. (WF-3): Optimization of Enzyme Production and Biochemical Characterization

Table 1

Effect of different pH (in vitro) on assay condition of α-galactosidase activity (U/mL).

Buffer	pH	Enzyme activity at the 5th day of culture incubation	Enzyme activity at the 6th day of culture incubation
Citrate	3	4.221 ± 0.92	39.66 ± 2.42
	4.6	4.470 ± 0.88	172.85 ± 8.31
Tris(glycine)	3	2.603 ± 0.72	21.11 ± 1.23
		3.130 ± 0.33	16.34 ± 0.98
Citrate phosphate	5	2.980 ± 0.22	48.80 ± 3.90
	6	5.578 ± 0.45	176.73 ± 9.12
	6.6	5.141 ± 0.42	57.56 ± 4.92
	7.4	5.540 ± 0.43	45.98 ± 3.92
Glycine (NaOH)	9.6	2.764 ± 0.32	122.43 ± 11.93
	10	2.603 ± 0.22	112.46 ± 10.77