Table of Contents
Biotechnology Research International
Volume 2016 (2016), Article ID 5030589, 11 pages
http://dx.doi.org/10.1155/2016/5030589
Research Article

The Use of Adenovirus Dodecahedron in the Delivery of an Enzymatic Activity in the Cell

1Centre National Recherche Scientifique, Université Joseph Fourier, European Molecular Biology Laboratory (Unit of Virus Host Cell Interactions, Unité Mixte Internationale-3265), 38042 Grenoble, France
2Faculty of Pharmacy, Hasanuddin University, Makassar 90245, Indonesia
3Institut de Biologie Structurale, 71 rue des Martyrs, 38027 Grenoble, France

Received 12 November 2015; Revised 18 January 2016; Accepted 28 January 2016

Academic Editor: Gabriel A. Monteiro

Copyright © 2016 Sumarheni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Penton-dodecahedron (Pt-Dd) derived from adenovirus type 3 is a symmetric complex of pentameric penton base plus fiber which can be produced in the baculovirus system at a high concentration. The size of Pt-Dd is smaller than the virus, but this virus-like particle (VLP) has the major proteins recognized by specific receptors on the surface of almost all types of cell. In this study, by direct observation with fluorescence microscopy on a fixed and living cell, the intracellular trafficking and localization of Pt-Dd labeled with fluorescence dyes in the cytoplasm of HeLa Tub-GFP showed a rapid internalization characteristic. Subsequently, the linkage of horseradish peroxidase (HRP) with Pt-Dd as the vector demonstrated an efficient system to deliver this enzyme into the cell without interfering its enzymatic activity as shown by biochemical and cellular experiments. These results were supported by additional studies using Bs-Dd or free form of the HRP used as the control. Overall, this study strengthens the potential role of Pt-Dd as an alternative vector for delivering therapeutic agents.