The Use of Adenovirus Dodecahedron in the Delivery of an Enzymatic Activity in the Cell

Figure 3

Colocalization of Pt-Dd in HeLa cell. (a) HeLa cells were incubated for 1 h with Alexa 555 labeled Pt-Dd (in yellow), caveolin was counterstained by anti-caveolin-1, and nuclei were counterstained by DAPI (in blue). (b) HeLa cell was incubated with Alexa 488 labeled Pt-Dd (in green) for 1 h and lysosomes were counterstained by LysoTracker (in orange). The pictures were taken on fluorescence microscopy (Olympus IX81-UCB) equipped with 60x oil-immersion objective lens using the according filters or differential interference contrast (DIC). Arrows indicate examples of colocalization.

(a)

(b)