Table of Contents
Chemotherapy Research and Practice
Volume 2011, Article ID 218750, 7 pages
http://dx.doi.org/10.1155/2011/218750
Research Article

Antibacterial and Antioxidant Properties of the Methanolic Extract of the Stem Bark of Pteleopsis hylodendron (Combretaceae)

1Laboratory of Microbiology and Antimicrobial Substances, University of Dschang, P.O. Box 67 Dschang, Cameroon
2Department of Biochemistry, University of Douala, P.O. Box 24157 Douala, Cameroon
3Laboratory of Phytochemistry, Institute of Medical Research and Medicinal Plants Study, Ministry of Scientific Research and Innovation, P.O. Box 6163, Yaoundé, Cameroon
4Department of Biochemistry, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon

Received 27 October 2010; Accepted 17 January 2011

Academic Editor: Athanassios Tsakris

Copyright © 2011 Aristide Laurel Mokale Kognou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pteleopsis hylodendron (Combretaceae) is used in Cameroon and West Africa folk medicine for the treatment of various microbial infections (measles, chickenpox, and sexually transmitted diseases). The antibacterial properties of the methanolic extract and fractions from stem bark of Pteleopsis hylodendron were tested against three Gram-positive bacteria and eight Gram-negative bacteria using Agar-well diffusion and Broth microdilution methods. Antioxidant activities of the crude extract and fractions were investigated by DPPH radical scavenging activity and β-carotene-linoleic acid assays. The methanolic extract and some fractions exhibited antibacterial activities that varied between the bacterial species (ID = 0.00–25.00 mm; MIC = 781–12500 μg/mL and 0.24–1000 μg/mL). The activity of the crude extract is, however, very weak compared to the reference antibiotics (MIC = 0.125–128 μg/mL). Two fractions (FE and FF) showed significant activity (MIC = 0.97 μg/mL) while S. aureus ATCC 25922 was almost resistant to all the tested fractions. In addition, the crude extract and some fractions showed good antioxidant potential with inhibition values ranging from 17.53 to 98.79%. These results provide promising baseline information for the potential use of this plant as well as some of the fractions in the treatment of infectious diseases and oxidative stress.