Methodology for Anti-Gene Anti-IGF-I Therapy of Malignant Tumours

Figure 2

Expression of IGF-I in primary human glioma cell line. RT PCR technique. M—marker. 1—presence of IGF-I in parental non transfected cells (200 bp band of amplified DNA using IGF-I primer; see Methods). 2—absence of IGF-I expression in cells transfected with antisense anti-IGF-I vector. 3—absence of IGF-I expression in cells transfected with triple helix anti-IGF-I vector.