Methodology for Anti-Gene Anti-IGF-I Therapy of Malignant Tumours

Figure 5

Expression of IGF-I in human cancer cell lines. RT PCR technique. M—marker. Lines 1 to 6—presence of IGF-I in parental nontransfected cancer cell lines derived from 1—glioblastoma, 2—hepatocarcinoma, 3—colon adenocarcinoma, 4—ovary cyst adenocarcinoma, 5—uterus endometrial adenocarcinoma, and 6—prostate adenocarcinoma (200 bp band of amplified DNA using IGF-I primer; see Section 2. Lines 7 to 12—absence of IGF-I expression in cancer cells transfected with both antisense and triple helix anti-IGF-I vectors: 7—glioblastoma, 8—hepatocarcinoma, 9—colon adenocarcinoma, 10—ovary cyst adenocarcinoma, 11—uterus endometrial adenocarcinoma, and 12—prostate adenocarcinoma.