Inhibition of NF-κB by Dehydroxymethylepoxyquinomicin Suppresses Invasion and Synergistically Potentiates Temozolomide and γ-Radiation Cytotoxicity in Glioblastoma Cells
Figure 2
(a) Treatment with DHMEQ (20 µg/mL) significantly increased apoptosis in 5 out of 6 cell lines. Cells were treated for 48 h and caspase-3 activation (Nucview 488 kit) was measured by fluorescent microscopy. Comparable levels of necrosis-like cell death (detected by differential staining with propidium iodide) were observed after treatment with the same dose after the same period. Data represents three independent experiments and are expressed as mean ± SD (). (b) Human GBM cells were exposed to DHMEQ (5 and 10 μg/mL) for 24 h at which time RNA was collected and used for qRT-PCR for the apoptosis-related genes BCL-XL, BCL2, and XIAP. Data represents two independent experiments in duplicate and are expressed as mean ± SEM.
