Inhibition of NF-κB by Dehydroxymethylepoxyquinomicin Suppresses Invasion and Synergistically Potentiates Temozolomide and γ-Radiation Cytotoxicity in Glioblastoma Cells
Figure 4
(a) DHMEQ potently abrogated the retained capacity for producing progeny of all GBM cell lines as detected by the clonogenic assay after 48 h of treatment. Each value represents the mean derived from at least three individual experiments (mean ± SD); (b) all cell lines presented a significant decrease in invasion potential as demonstrated by wound healing assay (significant after treatment with 10 μg/mL) and Matrigel-coated chambers (significant at all concentrations tested). Each value represents the mean derived from at least three individual experiments (mean ± SD); (c) migration-associated gene expression was also diminished in (4 out of 6) human GBM cells. In this case, cells were analyzed after treatment with 5 and 10 μg/mL of DHMEQ for 24 h in the presence of 1% FBS. Results are presented as normalized relative expression levels compared with control (DMSO) samples. Data represents two independent experiments in duplicate and are expressed as mean ± SEM.
