Modulators of Protein Kinase C Affect SR-BI-Dependent HDL Lipid Uptake in Transfected HepG2 Cells
Figure 5
SR-BI-ΔC retains DiI-HDL lipid uptake activity. HepG2 cells and stably transfected HepG2[eGFP-SR-BI] and HepG2[eGFP-SR-BI-ΔC] cells were incubated with DiI-HDL and analyzed by flow cytometry for DiI fluorescence (a) or eGFP fluorescence (b). The specific uptake of DiI lipid is shown in panel (a). The geometric mean eGFP fluorescence for HepG2[eGFP-SR-BI] and HepG2[eGFP-SR-BI-ΔC] cells is shown in panel (b). The experiment was performed in triplicate, and error bars represent standard deviation. by the Student’s -test.
