Figure 2: Effect of simvastatin on cholesterol levels in HCD-fed zebrafish larvae. Zebrafish larvae were fed a HCD with simvastatin mixed into zebrafish food at indicated quantities (μg simvastatin per gram food) for 14 days. Total cholesterol was measured in lipid extracts from larvae homogenates. Values of total cholesterol per larva were normalized to the values in the group that received HCD only (second column). Homogenates of 20 animals were pooled together for each data point in each individual experiment. Experiments with 0, 10, and 50 μg/g simvastatin were repeated twice, and average values from these two experiments are presented in the graph.