Figure 3: Effect of ezetimibe on cholesterol levels in HCD-fed zebrafish larvae. Zebrafish larvae were fed a HCD for 14 days. Ezetimibe was added at indicated concentrations directly to fish tank water, and the water was changed every day. Total cholesterol was measured in lipid extracts from larvae homogenates. Values of total cholesterol per larva were normalized to the values in the group that received HCD only (second column). Homogenates of 20 animals were pooled together for each data point in each individual experiment. Experiments with 0, 1, and 5 μM ezetimibe were repeated three times, and average values from these three experiments are presented in the graph.