The Influence of an Obesogenic Diet on Oxysterol Metabolism in C57BL/6J Mice
Figure 2
(a) FPLC lipoprotein profiles from mice after normal chow diet (solid line) and HFC diet (dashed line). Total lipoproteins were isolated by ultracentrifugation and lipoproteins were separated over two Superose 6 columns. Fractions 15–19 contain VLDL, 21–24 contain LDL, 25–27 contain LDL/HDL1, and HDL is found in fractions 29–34. (b) Western blot analysis of apo B, apo E, and apo AI in the pooled (six animals per group) FPLC fractions 25–27 (LDL/HDL1).