Table of Contents
Chinese Journal of Biology
Volume 2014, Article ID 205731, 12 pages
Research Article

Production of Extracellular Polymeric Substances by Halophilic Bacteria of Solar Salterns

Microbiology Laboratory, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, India

Received 13 May 2014; Accepted 15 July 2014; Published 6 August 2014

Academic Editor: Yinguang Chen

Copyright © 2014 Jhuma Biswas and A. K. Paul. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Moderately halophilic aerobic bacteria were isolated from 31 soil and 18 water samples collected from multipond solar salterns of Gujarat, Orissa, and West Bengal, India. A total of 587 bacterial isolates with distinct morphological features were obtained from these samples following dilution and plating on MH agar medium supplemented with NaCl. The isolates were screened for growth associated extracellular polymeric substances (EPS) production in MY medium under batch culture. In all, 20 isolates were selected as potent ones producing more than 1 g/L of EPS. These EPS producing isolates were characterized in detail for their morphological, physiological, and biochemical features and tentatively identified as members belonging to the genera Halomonas, Salinicoccus, Bacillus, Aidingimonas, Alteromonas, and Chromohalobacter. Apart from EPS production, these isolates also hold promise towards the production of various biomolecules of industrial importance.