Mimosine As Well As Serum Starvation Can Be Used for Cell Cycle Synchronization of Sheep Granulosa Cells

Table 1

Percentages (±SD) of granulosa cells in various phases of the cell cycle after a variety of culture treatments in primary culture (a) and 5th passage (b).

(a)

Treatment	G0/G1	S	G2/M
Normally growing	56.4 ± 2.7^a	19.0 ± 0.5^a	23.3 ± 2.5^f
Confluency	73.11 ± 3.0^bc	8.81 ± 1.41^bc	18.08 ± 0.58^a
24 h SS	76.9 ± 1.6^bc	7.8 ± 0.3^bc	15.3 ± 0.9^a
48 h SS	83.5 ± 1.3^bd	2.4 ± 0.1^bd	14.1 ± 0.6^bc
72 h SS	89.46 ± 2.0^bd	1.9 ± 0.3^bd	8.64 ± 1.14^bd
Mimosine	85.2 ± 1.1^bd	3.5 ± 0.3^bd	11.3 ± 0.6^be
Nocodazole	74.09 ± 1.07^bd	2.7 ± 0.5^bd	23.2 ± 1.7^abf

(b)

Treatment	G0/G1	S	G2/M
Normally growing	56.1 ± 1.0^a	18.0 ± 0.2^a	24. 8 ± 1.5^a
Confluency	72.6 ± 1.0^bc	6.5 ± 0.4^bd	20.9 ± 1.4^a
24 h SS	75.4 ± 2.2^bc	4.9 ± 0.4^bd	19.7 ± 0.5^a
48 h SS	85.7 ± 2.3^bd	2.1 ± 0.2^bc	12.2 ± 0.6^b
72 h SS	90.7 ± 1.72^bd	1.8 ± 0.45^bc	7.32 ± 0.42^b
Mimosine	85.5 ± 1.3^bd	4.5 ± 0.15^bd	10.0 ± 0.5^b
Nocodazole	74.6 ± 2.3^b	1.9 ± 0.56^bc	23.5 ± 1.8^a

SS: Serum starvation. Data are means ± SD. Values within the columns with different superscripts (a with b; c with d; e with f) differ significantly (𝑃<0.05).