Table of Contents
Chinese Journal of Biology
Volume 2015, Article ID 309267, 6 pages
http://dx.doi.org/10.1155/2015/309267
Research Article

Bacterial Endo-Symbiont Inhabiting Tridax procumbens L. and Their Antimicrobial Potential

1Herbal Drugs Technological Laboratory, Department of Studies in Microbiology, Manasagangotri, University of Mysore, Mysore, Karnataka 570 006, India
2Department of Zoology, Bangalore University, Jnanabharathi Campus, Bangalore, Karnataka 560 056, India
3Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden
4Department of Plant Pathology, University of Georgia, Athens, GA 30602, USA

Received 22 August 2015; Revised 23 October 2015; Accepted 28 October 2015

Academic Editor: Rajesh Jeewon

Copyright © 2015 Syed Baker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bacterial symbionts inhabiting Tridax procumbens L. were screened for antimicrobial potential with the aim to isolate potent bacteria bearing significant activity against test pathogens. The selected isolate was subjected to large scale fermentation to extract antimicrobial metabolite. The organic phase was reduced under vacuum pressure and crude ethyl acetate extract (10 mg/mL) was evaluated for antimicrobial activity against panel of test pathogens. The antibacterial activity was measured as a zone of inhibition and compared with standard antibiotics, gentamicin and tetracycline. Similarly, antifungal activity was compared with miconazole and bavistin. Significant activity was conferred against Shigella flexneri (MTCC 731) with  mm zone across the disc. Partially, purification of antimicrobial metabolite with TLC-bioautography and HPLC resulted in active fraction bearing activity at 0.65 and eluting between 4 and 5 retention times. The obtained results are promising enough for future purification and characterization of antimicrobial metabolite. Thus, the study attributes to the growing knowledge on endophytes as one of the rich sources of antimicrobial potentials.