Table of Contents
Chinese Journal of Biology
Volume 2015 (2015), Article ID 686071, 10 pages
http://dx.doi.org/10.1155/2015/686071
Research Article

Hepatoprotective Effect of Citrus limon Fruit Extract against Carbofuran Induced Toxicity in Wistar Rats

1Department of Biochemistry, University of Allahabad, Allahabad 211002, India
2Department of Biochemistry, King Saud University, Riyadh, Saudi Arabia
3Department of Zoology, University of Allahabad, Allahabad 211002, India

Received 26 August 2015; Accepted 4 October 2015

Academic Editor: Yinguang Chen

Copyright © 2015 Sunil Kumar Jaiswal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranol methylcarbamate), is known to induce oxidative stress and to cause inhibition of acetylcholinesterase activity. The present work was envisaged to evaluate the effect of carbofuran on redox indices and its interactions with hepatic markers in rat. The ameliorating effect of Citrus limon fruit extract on carbofuran induced toxicity was also monitored. The results indicated that carbofuran treatment caused significant alterations in the levels of activities of AST, ALT, and LDH in liver tissues and serum. The levels of enzymatic oxidative stress markers such as SOD and catalase and nonenzymatic redox molecules such as total thiol, GSH, and protein thiol also showed significant perturbations in rat liver due to carbofuran treatment. The administration of Citrus limon fruit extract, however, was able to markedly ameliorate the toxicity of carbofuran by protecting the levels of aforesaid biomarkers to near normal levels. The ameliorative effect of Citrus limon fruit extract may be due to the presence of different antioxidants in it which may neutralize the ROS and RNS generated in the body tissue due to pesticide stress. These results suggested that Citrus limon fruit extract may be utilized as a potential supplement in proper management of pesticide intoxication in association with relevant therapeutics.