Immune Regulation and Oxidative Stress Reduction by Preimplantation Factor following Syngeneic or Allogeneic Bone Marrow Transplantation
Figure 4
PIF regulates immune response through monocytes. (a) FACS analysis of bone marrow cells (upper panel) and blood cells (lower panel) stained with FITC-labeled PIF or PIFscr and antibodies against CD3 (T-cell marker), CD19 (B-cell marker), and CD11b (monocytes and granulocytes marker). The % of specific binding is the % of PIF binding cells minus % of PIFscr (nonspecific) binding cells; summary of 2 experiments. (b) Thymidine proliferation assay of mouse T cells in co-culture with bone marrow-derived monocytes treated with PIF; summary of 4 experiments. The results are shown as % of control. (Mann-Whitney: ). (c) Nitric Oxide secretion from RAW cells treated/nontreated with PIF for 3 days and activated with LPS for 24 h. One representative experiment is shown out of three performed. (d) iNOS mRNA expression in RAW 264.7 cells treated/nontreated with PIF for the indicated and activated with LPS for 24 h.
