The GRK2 Overexpression Is a Primary Hallmark of Mitochondrial Lesions during Early Alzheimer Disease
Figure 5
The GRK2 immunoreactivity in rat brain hippocampal tissues was exposed to 2-vessel occlusion and determined by using preembedding immunogold cytochemistry techniques. (a) Subcellular determination of GRK2 in the neuronal cell body shows the presence of GRK2 immunopositive gold particles (arrows), which associates with the external membrane and the matrix of damaged but not intact mitochondria (M), X 40,000. (b) Neurons containing granular vacuolar degenerative structures show island of GRK2 immunopositive gold particles (single arrow), X 30,000. (c) The glial cell body shows overexpression of GRK2 immunoreactivity in the matrix of granular vacuolar degenerative structures (single arrow), X 50,000. (d) Neurofilament from the damaged neurons shows the presence of GRK2 immunopositive gold particles (single arrows), X 40,000 (reprinted with permission of Neurotoxicity Research [8]).
