Pharmacological Characterization of Inositol 1,4,5-tris Phosphate Receptors in Human Platelet Membranes
Figure 6
Effects of CaCl2 on [3H]Ins(1,4,5)P3 binding to human platelet membranes in the presence (a) and absence (b) of EDTA. Platelet membranes were incubated with increasing concentrations of CaCl2 in the presence of 20 nM [3H]Ins(1,4,5)P3 in a buffer containing 50 mM Tris-HCl, pH 8.4; 1 mM EDTA; and 1 mM 2-mercaptoethanol at 4°C for 10 minutes. The data represent the inhibition or stimulation of [3H]Ins(1,4,5)P3 binding to platelet membranes. The points represent the mean S.E.M. of three independent experiments, each run in duplicate.
