Table of Contents
Cardiovascular Psychiatry and Neurology
Volume 2010, Article ID 720958, 6 pages
Research Article

A Comparison of the Dynamics of S100B, S100A1, and S100A6 mRNA Expression in Hippocampal CA1 Area of Rats during Long-Term Potentiation and after Low-Frequency Stimulation

1Institute for Molecular Biology and Biophysics, Siberian Branch of the Russian Academy of Medical Sciences, 2 Timakova Street, Novosibirsk 630117, Russia
2Laboratory of Biomedical Informatics, Design Technological Institute of Digital Techniques, Siberian Branch of The Russian Academy of Sciences, 6 Institutskaya ul., Novosibirsk 630090, Russia
3Research Division, OOO NPF Materia Medica Holding, 9 3rd Samotyochnyi per., Moscow 127473, Russia

Received 1 March 2010; Revised 27 April 2010; Accepted 17 August 2010

Academic Editor: Rosario Donato

Copyright © 2010 Pavel D. Lisachev et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The interest in tissue- and cell-specific S100 proteins physiological roles in the brain remains high. However, necessary experimental data for the assessment of their dynamics in one of the most important brain activities, its plasticity, is not sufficient. We studied the expression of S100B, S100A1, and S100A6 mRNA in the subfield CA1 of rat hippocampal slices after tetanic and low-frequency stimulation by real-time PCR. Within 30 min after tetanization, a 2–4 fold increase of the S100B mRNA level was observed as compared to the control (intact slices) or to low-frequency stimulation. Subsequently, the S100B mRNA content gradually returned to baseline. The amount of S100A1 mRNA gradually increased during first hour and maintained at the achieved level in the course of second hour after tetanization. The level of S100A6 mRNA did not change following tetanization or low-frequency stimulation.