The Calcium-Dependent Interaction of S100B with Its Protein Targets

Figure 8

Target protein binding profiles for S100 family members. Membranes containing glycogen phosphorylase (a) (Gpa), glycogen phosphorylase (b) (Gpb), phosphoglucomutase (PGM), and tau (50 pmoles) were incubated in 100 nM Alexa Flour 488 labeled S100B (blue bars), S100A1 (red bars), S100P (green bars), S100A4 (brown bars), and S100A5 (yellow bars) in the presence (darker bars) or absence (lighter bars) of Ca²⁺. The histograms depict that the mean pmoles S100 bound ± the SEM and N’s denote no detectable binding. Asterisks denote P ≤  .05 between the ±Ca²⁺ conditions.

(a)

(b)