Intercellular Interactomics of Human Brain Endothelial Cells and Th17 Lymphocytes: A Novel Strategy for Identifying Therapeutic Targets of CNS Inflammation

Figure 4

Extracellular signaling (communication) between Th17 and BEC. Shown is a visual depiction of the intercellular interaction network between proteins secreted by either Th17 lymphocytes or BEC and their interacting membrane counterparts expressed on the other cell type. All identified membrane proteins are represented by ovals, whereas secreted proteins are represented as circles: in red are luminal BEC membrane proteins that interact with secreted T17 proteins (in blue circles); in blue are membrane T17 proteins that interact with secreted BEC proteins (in red circles). Proteins were identified using membrane and subcellular proteomics and glycoproteomics and their interactions were catalogued using protein-protein databases as described in the text (each interaction is shown as a line connecting interacting proteins).