Elevated Serum C-Reactive Protein Relates to Increased Cerebral Myoinositol Levels in Middle-Aged Adults
C-reactive protein (CRP), a systemic marker of inflammation, is a risk factor for late life cognitive impairment and dementia, yet the mechanisms that link elevated CRP to cognitive decline are not fully understood. In this study we examined the relationship between CRP and markers of neuronal integrity and cerebral metabolism in middle-aged adults with intact cognitive function, using proton magnetic resonance spectrocospy. We hypothesized that increased levels of circulating CRP would correlate with changes in brain metabolites indicative of early brain vulnerability. Thirty-six individuals, aged 40 to 60, underwent neuropsychological assessment, a blood draw for CRP quantification, and 1H MRS examining N-acetyl-aspartate, myo-inositol, creatine, choline, and glutamate concentrations in occipito-parietal grey matter. Independent of age, sex and education, serum CRP was significantly related to higher cerebral myo-inositol/creatine ratio (), a relationship which remained unchanged after adjustment for cardiovascular risk (, CRP β = 0.322, ). Because these biomarkers are detectable in midlife they may serve as useful indicators of brain vulnerability during the preclinical period when mitigating intervention is still possible.
Much evidence suggests that inflammatory conditions common in midlife such as obesity, atherosclerosis, and Type II diabetes play an important role in the development of dementia [1, 2]. Longitudinal studies have indicated that low-grade systemic inflammation in middle age adds to the risk of late-life cognitive impairment over and above the risk assessment afforded by disease (e.g., hypertension and atherosclerosis) and lifestyle habits (e.g., smoking) [1, 3, 4]. In addition, increases in serum inflammatory markers such as interleukin-6 (IL-6) and C-reactive protein (CRP) have been shown to exacerbate cognitive decline in older adults with metabolic syndrome, a condition defined by cooccurring obesity, hypertension, dyslipidemia, and hyperglycemia [2, 5]. The long-term use of anti-inflammatory drugs (NSAIDS), on the other hand, has been associated with a reduction in dementia risk [6–8], lending further support to the idea that sustained activation of an inflammatory immune response can foster neuronal vulnerability beyond what is expected of normal aging.
With increasing age, inflammatory changes are appreciable in the brain in the form of astrocyte proliferation and the presence of activated microglia . However, it is still unclear in what capacity midlife inflammation initiates detectable changes in the brain that, if left unchecked, may progress or contribute to neurodegenerative conditions. Such information will be invaluable as chronic subclinical inflammation presents a potentially modifiable risk factor for future cognitive decline and dementia. Early detection of biomarkers linked to cognitive decline allows time for mitigating action to be taken, prior to the onset of irreversible clinical signs.
With this information as background, our goal was to investigate the relationship between systemic inflammation and cerebral metabolism in middle-aged adults with intact cognitive function. Specifically, we examined the association between serum levels of CRP and markers of neuronal integrity, osmolarity, and glial proliferation measured by magnetic resonance spectroscopy (1H MRS). To our knowledge, this is the first study to examine the relationship between an inflammatory marker and cerebral metabolites in cognitively healthy adults. As such, it offers novel information regarding the cooccurrence of increasing systemic inflammation and changes in neurochemistry within a middle-aged sample.
CRP is an acute phase protein released by hepatocytes in response to increases in circulating inflammatory cytokines . CRP also serves as an activator of the complement system, an immunological cascade that assists the innate immune system by marking and destroying non-self antigens. Activation of complement is thought to contribute to the perpetuation of the inflammatory response and is implicated in neurodegenerative processes .
Perturbations in the levels of certain cerebral metabolites have been correlated with a variety of clinical conditions. Two markers in particular have been directly linked to changes in cognitive functioning: N-acetylaspartate (NAA) and Myoinositol (mI). NAA occurs exclusively in neurons and oligodendrocyte-type-2 astrocytes  and is widely regarded as a marker of neuronal viability, synaptic health, and metabolism . Higher levels of NAA are linked to better cognitive test performance , and reduced levels are associated with cognitive impairment following brain injury  and neurodegenerative diseases . Myoinositol (mI) is an organic osmolyte and purported glial marker . Changes in levels of mI have been shown to presage the onset of cognitive decline in conditions fostering neuroinflammation, such as HIV and Alzheimer’s disease [18–21]. Elevations of mI levels in Alzheimer’s disease have been interpreted as a sign of gliosis .
We hypothesized that increased levels of circulating CRP would correlate with changes in brain metabolites indicative of early brain vulnerability, such as lower levels of NAA and/or higher levels of mI.
2. Materials and Methods
The study was approved by the Institutional Review Board of the University of Texas at Austin, and all volunteers provided written informed consent before enrollment. Participants were required to complete a medical history interview in which medical conditions and treatments were coded as either present or absent based on participants’ self-report. Participants then underwent a full neuropsychological evaluation and a general health assessment, including a fasting blood draw for CRP. Visits were conduced on separate days and participants completed the study within one month.
Participants between the ages of 40 and 60 were recruited through flyers and newspaper advertisements. Exclusion criteria included a history of neurological disease (e.g., large vessel stroke, seizure disorder, Parkinson’s disease, clinically significant traumatic brain injury, multiple sclerosis, or brain infection/meningitis), major psychiatric illness (e.g., schizophrenia, bipolar disorder), substance abuse (diagnosed abuse and/or previous hospitalization for substance abuse), diabetes mellitus, serum CRP greater than 10 mg/L, or MRI contraindications. One hundred and three volunteers were screened; of these, 36 participants (14 men, 22 women) passed the initial screening process and were included in the final sample.
The mean (±SD) age of the sample was years. The mean education level was years. The mean full-scale IQ score was , indicating high average global cognitive functioning according to published norms . Enrollees identified themselves as follows: 42% Caucasian, 39% Hispanic, 3% Asian, 11% African American, and 6% Other.
2.2.1. General Health Screening
Fasting blood concentrations of glucose, triglyceride, HDL-cholesterol, and LDL-cholesterol were determined using the standard enzymatic technique. Arterial blood pressure was measured using a standard oscillometric blood pressure monitor (VP-2000, Colin Medical Instruments, San Antonio, TX) after at least 15 minutes of rest. Body Mass Index (BMI) was calculated as weight in kilograms divided by the square of height in meters. Since CRP is also a recognized cardiovascular risk factor , and cardiovascular disease is known to impact cognition [2, 25, 26], cardiovascular disease (CVD) risk factors (including obesity, hypertension, dyslipidemia, hyperglycemia, and history of smoking) were coded as present or absent (1 or 0) based on the American Heart Association recommendations for risk criteria . These risk factors were then summed to create an overall CVD risk score (range 0–5) that was included as a covariate in subsequent analyses.
2.2.2. High Sensitivity C-Reactive Protein (CRP) Assay
A 3 mL fasting blood sample was collected from the antecubital vein by venipuncture. Serum was separated within 2 hours of the collection, and aliquots were stored at −80°C until later analysis. Prior to the analysis, the serum sample was diluted 100-fold. Serum concentration of CRP was measured using high sensitivity human ELISA kits (Alpha Diagnostics, San Antonio, TX) with a minimum detectable concentration of 0.35 ng/mL.
2.2.3. Neuropsychological Evaluation
All participants completed a two-hour assessment battery including standard clinical neuropsychological instruments with established reliability and validity . Neuropsychological measures were grouped into one of five cognitive domains: (1) global cognitive functioning, (2) language functions, (3) memory functions (4) attention-executive-psychomotor functions, and (5) visual-spatial abilities. The following test scores were included in each domain, and raw total scores were utilized: (1) global: WASI Full Scale IQ  and Mini-Mental State Examination (MMSE) ; (2) language: WASI Vocabulary Subtest and Category Fluency for Animals ; (3) memory: CVLT-II immediate recall, delayed recall, recognition discrimination , Rey Complex Figure Test (RCF) immediate recall, delayed recall, and recognition discrimination ; (4) attention-executive-psychomotor functioning: WAIS-III, Digit Span Subtest , Controlled Oral Word Association Test, (COWAT) , Trail Making Test A and B , and Grooved Pegboard-Dominant Hand time to completion ; (5) visual-spatial abilities: RCF copy and WASI Matrix Reasoning Subtest. Emotional functioning was also assessed with the Beck Depression Inventory-II (BDI)  and the Trait Anxiety Inventory (STAI-T) . All tests were administered and scored by a trained research assistant using standard administration and scoring criteria. Participants’ raw test scores were converted to z-scores using the study sample mean and standard deviation. Timed test scores were multiplied by −1 so that higher scores indicate better performance. Five composite cognitive domain z-scores were calculated for each participant by averaging the z-scores of all tests within that domain.
Structural imaging included a high-resolution Spoiled Gradient Echo (SPGR) sequence ( matrix, FOV = cm2, 1 mm slice thickness, 0 gap) anatomical scan of the entire brain in the sagittal plane. 1H MRS data for each participant were acquired in a single session on a 3T GE Signa Excite MRI scanner equipped with a standard head coil. Single voxel proton 1H MRS was performed using the automated GE pulse sequence PROBE-P, which is a point resolved spectroscopy (PRESS) sequence with chemical shift selected (CHESS) water suppression. 1H-MRS parameters were as follows: echo time/repetition time (TE/TR) = 35/3000 ms, 128 excitations, 5000 Hz spectral width, volume ~6 cm3 from the occipitoparietal gray matter (Figure 1). The region was chosen because spectroscopically detectable alterations in its neurochemical composition are well documented in disorders of cognition, in correspondence with severity of cognitive dysfunction . Commercially available software, LCModel, was used to quantify and separate the metabolite resonances from the macromolecule background . In line with standard clinical protocols, the concentrations of NAA, choline containing compounds (choline, phosphocholine and glycerophosphocholine, Cho), myoinositol, and glutamate (Glu) were reported as ratios relative to creatine (Cr) [22, 39]. MRS data for all participants in the final sample met quality control criteria (Cramer-Rao Lower Bounds for NAA, mI, Cho or Glu <12).
Within pathological conditions, the use of Cr as a stable standard for comparison of other metabolites is debated, as are the benefits of reporting absolute versus fractional quantities of metabolites. We opted to report metabolite concentrations in ratio to Cr because our sample consists of healthy middle-aged individuals, and because ratios are more easily obtained during clinical scans and hence possess more clinical utility.
2.3. Data Analyses
The relationship between CRP and the 1H MRS markers (NAA/Cr, Glu/Cr, Cho/Cr and mI/Cr) was analyzed using a single multivariate multiple linear regression model with all MRS parameters entered in at once. Age, sex, and level of education were chosen as covariates a priori, since they are known to influence the risk of cognitive vulnerability [40, 41]. Follow-up analyses of the relationship between mI/Cr and CRP were conducted using linear regression. All statistical analyses were performed using SPSS 16.0 (SPSS Inc., Chicago, IL). A two-tailed-alpha level of 0.05 was used as the criterion for statistical significance.
3.1. Selected Subject Characteristics
Selected characteristics of the subjects are reported in Table 1. Raw cognitive test scores and standard deviations are reported in Table 2. Descriptive statistical analyses revealed a cognitively normal, ethnically diverse, and middle-aged sample, well representative of the population of the state of Texas based on 2000 US census data for the state. Regression residuals were normally distributed according to the Shapiro-Wilk test of normality (Shapiro-Wilk > 0.95, ), and no transformations were performed.
3.2. Demographics and Cardiovascular Risk in relation to CRP and Cerebral Metabolism
CRP was not significantly related to age, sex, or education level. As expected, increased CRP was significantly related to CVD risk (, ). We quantified CVD risk as a 0–5 index score based on the current presence of each of the following risk factors: obesity, hypertension, dyslipidemia, hyperglycemia, and history of smoking. Among cerebral metabolites, male sex was correlated with higher mI/Cr (, ). No other cerebral metabolites reached statistical significance in relation to demographic variables. Similarly, no cerebral metabolites were significant relative to level of CVD risk. Among the variables used to assess CVD risk, obesity and elevated fasting glucose were each significantly associated with higher CRP (, , and , , resp.), whereas a history of smoking (, , ), dyslipidemia (, , ), and hypertension (, , ) were not.
3.3. Cognition in relation to CRP and Cerebral Metabolism
We found no significant relations between CRP and measures of global cognitive functioning (, ), language (, ), memory (, ), attention-executive-psychomotor functioning (, ), or visual-spatial ability (, ). Nor did we find significant relations between NAA/Cr, Cho/Cr, Glu/Cr, or mI/Cr and any domain of cognitive functioning. These findings were not surprising considering our relatively young, cognitively intact sample (average global cognitive function (FSIQ) = 112.3).
3.4. CRP in relation to Cerebral Metabolism
Relationships between CRP and cerebral metabolism were examined by multivariate multiple regression analyses adjusted for sex, age, and years of education. In fully adjusted analyses, CRP was positively associated with cerebral mI/Cr in the brain ((4,31) = 4.74, ) but not with NAA/Cr ((4,31) = 0.99, ), Cho/Cr ((4,31) = 0.53, ) or Glu/Cr ((4,31) = 1.64, ). Coefficients are reported in Table 3. This relationship remained unchanged even after adjustment for cardiovascular risk ((5,30) = 4.356, CRP , ).
We found that higher serum CRP, a sensitive marker of systemic inflammation, was significantly related to higher cerebral mI/Cr concentrations in cognitively normal middle-aged adults. This finding adds to the growing body of literature relating CRP to neurochemical changes linked to the development of cognitive impairment in later life [1, 25, 42–44]. It extends the previous literature by suggesting that systemic inflammation is related to neurochemical changes even in middle-aged adults with intact cognitive functioning.
We chose to examine CRP because it presents a stable, easily obtained inflammatory marker that is not subject to variation based on time of sampling or blood glucose levels . CRP is also a well-known risk factor for vascular disease and endothelial dysfunction [27, 46–49], both of which contribute to the rate and severity of cognitive decline over the lifespan [50, 51]. Perturbations in cerebral mI/Cr, an organic osmolyte and marker of glial proliferation , have also been shown to precede the onset of cognitive decline in conditions fostering immune activation and neuroinflammation [18–21]. However, until now, no studies have specifically looked for a correlation between cerebral mI/Cr levels and systemic inflammation. Our results indicate that increased systemic inflammation covaries with increased cerebral mI/Cr. While our study does not allow for inferences regarding a mechanistic linkage between CRP and changes in mI/Cr, it nonetheless highlights a potential relationship between these two factors. Importantly, this relationship is detectable in midlife, prior to clinically significant changes in cognitive status.
One explanation for the increased mI/Cr observed in our sample could be increased brain water diffusion in the presence of proinflammatory conditions. Because of its degrading effect on tissue integrity, inflammation has been positively correlated with increased white matter diffusion in neurodegenerative disease [52, 53], acute neuroinflammatory conditions , and normal aging . In turn, increased brain water diffusion has been linked to higher levels of cerebral osmolytes including mI/Cr . Systemic inflammation and resultant endothelial dysfunction could also contribute to proinflammatory conditions in the brain [2, 47], leading to increased brain water diffusion and glial accumulation of osmolytes such as mI/Cr . CRP in particular has been implicated in autotoxic cascades leading to decreased endothelial integrity , which over time may impair cerebrovascular autoregulation and negatively impact cognition .
Although our sample consisted of healthy adults without clinical signs of neuroinflammation, many of them were positive for one or more cardiovascular risk factors (e.g., hypertension, obesity) associated with increased circulating inflammatory markers and endothelial dysfunction. In particular, the mean BMI of our sample, 29.7 (SD = 5.6), is considered obese according to clinical guidelines defining obesity as a BMI equal to or greater than 30 kg/m2 . Abdominal obesity has been linked to elevated levels of CRP  and is understood as a causative factor for endothelial dysfunction . It is possible that the higher mean BMI observed in our sample is one factor contributing to endothelial dysfunction and accompanying changes in the neurochemical milieu.
Additionally, the mean CRP value of our sample (2.72 mg/L) falls just below the high-risk category for cardiovascular disease events according to the American Heart Association . The American Heart Association’s CRP risk categories (<1.0 mg/L = low, 1.0 to 3.0 mg/L = average, and >3.0 mg/L = high) are based on CRP tertiles observed in the adult population. The high-risk tertile has roughly twice the relative risk of a cardiovascular disease event compared to the low-risk tertile . The mean CRP value obtained for our participants, especially taken together with the high mean BMI, indicates that our sample may be at greater risk for decreased endothelial integrity and increased brain water diffusion. However, it should be stressed again that the correlation between elevated CRP and elevated mI/Cr does not indicate a mechanistic linkage between the two, and the explanations offered for elevated mI/Cr in this sample remain hypothetical.
A related explanation for increased mI/Cr in our sample is increased blood brain barrier (BBB) permeability , which may contribute to neuronal vulnerability and eventual cognitive decline. Myoinositol accumulates preferentially in astrocytes, which, together with endothelial tight junctions, provide selective permeability of the BBB. BBB permeability increases in the presence of inflammatory markers and may lead to toxic elevations of intracellular calcium . In our sample increased levels of mI/Cr could be interpreted as an early sign of astrocyte proliferation related to impaired endothelial function and resultant osmotic stress in response to low-grade systemic inflammation. Support for this hypothesis comes from the upregulation of mI synthesis in the presence of conditions causing BBB permeability, such as trauma, carbon monoxide poisoning, demyelination, beta-amyloid deposits , and neurofibrillary tangles . These conditions represent extreme events leading to neuronal vulnerability and cell death and are not assumed to be present in our sample. However, taken together, our observed increases in mI/Cr and CRP could be viewed as two early indicators of neuronal vulnerability similar to though less extreme than the conditions above. Further support for this hypothesis comes from evidence that elevated mI/Cr precedes changes in the levels of other cerebral metabolites in diseases marked by cognitive decline, such as Alzheimer’s disease and other dementias .
The primary strength of our study was the thorough characterization of our sample in terms of cognitive and physiological functioning. The cognitive battery utilized in the study examined multiple domains of cognitive functioning and relied on at least two measures within each domain. Assessments of metabolic and vascular health were performed by trained researchers rather than relying on self-reported medical history, which may contain unintentional omissions and errors. External confirmation of cognitive and physiological health status has allowed us to interpret our findings with greater confidence, without the potentially confounding effects of underreported medical conditions or cognitive disorders.
The cross-sectional design of our study prevents determination of a causal link between inflammation and increased mI/Cr and demonstrates only that there may be a relationship between these two factors. It is highly likely that multiple factors in addition to these contribute to cognitive decline over the lifespan. Our small, well-educated sample also limits the generalizability of our findings. Moreover, we did not obtain any additional assessments of neuronal integrity, such as Diffusion Tensor Imaging, white matter hyperintensities, or additional markers of inflammation, such as IL-6. Future longitudinal studies beginning in midlife and conducted with larger, randomly selected community samples could help elucidate potential interactions between systemic inflammation and neural metabolism and the extent to which these factors predict cognitive decline. Additionally, combining multiple measures of neuronal health and multiple measures of inflammation would offer a more complete picture of the ways in which brain health is affected by systemic inflammation. Finally, we did not quantify other conditions known to correlate with CRP levels, such as alcohol consumption, stress, and sleep quality. Future studies should further examine these variables as they relate to CRP levels and changes in cerebral mI/Cr.
|mI/Cr:||the ratio of myo-inositol to creatine.|
This paper was supported by the American Heart Association (A.P.H., 09BGIA2060722); the American Federation for Aging Research (A.P.H., 8A0024); the University of Texas at Austin. The authors thank the Imaging Center Staff for their help with the participants.
R. Schmidt, H. Schmidt, J. D. Curb, K. Masaki, L. R. White, and L. J. Launer, “Early inflammation and dementia: a 25-year follow-up of the Honolulu-Asia Aging Study,” Annals of Neurology, vol. 52, no. 2, pp. 168–174, 2002.View at: Publisher Site | Google Scholar
K. Yaffe, A. Kanaya, K. Lindquist et al., “The metabolic syndrome, inflammation, and risk of cognitive decline,” JAMA, vol. 292, no. 18, pp. 2237–2242, 2004.View at: Publisher Site | Google Scholar
G. Ravaglia, P. Forti, F. Maioli et al., “Blood inflammatory markers and risk of dementia: the Conselice Study of Brain Aging,” Neurobiology of Aging, vol. 28, no. 12, pp. 1810–1820, 2007.View at: Publisher Site | Google Scholar
R. S. Tilvis, M. H. Kähönen-Väre, J. Jolkkonen, J. Valvanne, K. H. Pitkala, and T. E. Strandberg, “Predictors of cognitive decline and mortality of aged people over a 10-year period,” Journals of Gerontology—Series A, vol. 59, no. 3, pp. M268–M274, 2004.View at: Google Scholar
M. G. Dik, C. Jonker, H. C. Comijs et al., “Contribution of metabolic syndrome components to cognition in older individuals,” Diabetes Care, vol. 30, no. 10, pp. 2655–2660, 2007.View at: Publisher Site | Google Scholar
P. L. McGeer and E. G. McGeer, “NSAIDs and Alzheimer disease: epidemiological, animal model and clinical studies,” Neurobiology of Aging, vol. 28, no. 5, pp. 639–647, 2007.View at: Publisher Site | Google Scholar
P. L. McGeer, M. Schulzer, and E. G. McGeer, “Arthritis and anti-inflammatory agents as possible protective factors for Alzheimer's disease: a review of 17 epidemiologic studies,” Neurology, vol. 47, no. 2, pp. 425–432, 1996.View at: Google Scholar
C. A. Szekely, J. C. S. Breitner, A. L. Fitzpatrick et al., “NSAID use and dementia risk in the Cardiovascular Health Study: role of APOE and NSAID type,” Neurology, vol. 70, no. 1, pp. 17–24, 2008.View at: Publisher Site | Google Scholar
J. R. Conde and W. J. Streit, “Microglia in the aging brain,” Journal of Neuropathology and Experimental Neurology, vol. 65, no. 3, pp. 199–203, 2006.View at: Publisher Site | Google Scholar
D. C. W. Lau, B. Dhillon, H. Yan, P. E. Szmitko, and S. Verma, “Adipokines: molecular links between obesity and atheroslcerosis,” American Journal of Physiology, vol. 288, no. 5, pp. H2031–H2041, 2005.View at: Publisher Site | Google Scholar
D. M. Bonifati and U. Kishore, “Role of complement in neurodegeneration and neuroinflammation,” Molecular Immunology, vol. 44, no. 5, pp. 999–1010, 2007.View at: Publisher Site | Google Scholar
J. Urenjak, S. R. Williams, D. G. Gadian, and M. Noble, “Specific expression of N-acetylaspartate in neurons, oligodendrocyte-type- 2 astrocyte progenitors, and immature oligodendrocytes in vitro,” Journal of Neurochemistry, vol. 59, no. 1, pp. 55–61, 1992.View at: Publisher Site | Google Scholar
E. R. Danielsen and B. Ross, Magnetic Resonance Spectroscopy Diagnosis of Neurological Diseases, Marcel Dekker, New York, NY, USA, 1999.
R. E. Jung, R. A. Yeo, S. J. Chiulli, W. L. Sibbitt, and W. M. Brooks, “Myths of neuropsychology: intelligence, neurometabolism, and cognitive ability,” Clinical Neuropsychologist, vol. 14, no. 4, pp. 535–545, 2000.View at: Google Scholar
W. M. Brooks, S. D. Friedman, and C. Gasparovic, “Magnetic resonance spectroscopy in traumatic brain injury,” The Journal of Head Trauma Rehabilitation, vol. 16, no. 2, pp. 149–164, 2001.View at: Google Scholar
C. E. Clarke and M. Lowry, “Systematic review of proton magnetic resonance spectroscopy of the striatum in parkinsonian syndromes,” European Journal of Neurology, vol. 8, no. 6, pp. 573–577, 2001.View at: Publisher Site | Google Scholar
A. Brand, C. Richter-Landsberg, and D. Leibfritz, “Multinuclear NMR studies on the energy metabolism of glial and neuronal cells,” Developmental Neuroscience, vol. 15, no. 3–5, pp. 289–298, 1993.View at: Google Scholar
W. Huang, G. E. Alexander, E. M. Daly et al., “High brain myo-inositol levels in the predementia phase of Alzheimer's disease in adults with Down's syndrome: a 1H MRS study,” American Journal of Psychiatry, vol. 156, no. 12, pp. 1879–1886, 1999.View at: Google Scholar
S. Chantal, M. Labelle, R. W. Bouchard, C. M. J. Braun, and Y. Boulanger, “Correlation of regional proton magnetic resonance spectroscopic metabolic changes with cognitive deficits in mild Alzheimer disease,” Archives of Neurology, vol. 59, no. 6, pp. 955–962, 2002.View at: Google Scholar
C. C. Cloak, L. Chang, and T. Ernst, “Increased frontal white matter diffusion is associated with glial metabolites and psychomotor slowing in HIV,” Journal of Neuroimmunology, vol. 157, no. 1-2, pp. 147–152, 2004.View at: Publisher Site | Google Scholar
K. T. M. Fernando, M. A. McLean, D. T. Chard et al., “Elevated white matter myo-inositol in clinically isolated syndromes suggestive of multiple sclerosis,” Brain, vol. 127, no. 6, pp. 1361–1369, 2004.View at: Publisher Site | Google Scholar
K. Kantarci, C. R. Jack, Y. C. Xu et al., “Regional metabolic patterns in mild cognitive impairment and Alzheimer's disease: a 1H MRS study,” Neurology, vol. 55, no. 2, pp. 210–217, 2000.View at: Google Scholar
D. Wechsler, Wechsler Abbreviated Scale of Intelligence Manual, Harcourt Assessment Company, San Antonio, Tex, USA, 1999.
P. M. Ridker, “C-Reactive protein and the prediction of cardiovascular events among those at intermediate risk—moving an inflammatory hypothesis toward consensus,” Journal of the American College of Cardiology, vol. 49, no. 21, pp. 2129–2138, 2007.View at: Publisher Site | Google Scholar
K. Yaffe, K. Lindquist, Penninx et al., “Inflammatory markers and cognition in well-functioning African-American and white elders,” Neurology, vol. 61, no. 1, pp. 76–80, 2003.View at: Google Scholar
R. A. Whitmer, S. Sidney, J. Selby, S. Claiborne Johnston, and K. Yaffe, “Midlife cardiovascular risk factors and risk of dementia in late life,” Neurology, vol. 64, no. 2, pp. 277–281, 2005.View at: Google Scholar
T. A. Pearson, G. A. Mensah, R. W. Alexander et al., “Markers of inflammation and cardiovascular disease: application to clinical and public health practice: a statement for healthcare professionals from the centers for disease control and prevention and the American Heart Association,” Circulation, vol. 107, no. 3, pp. 499–511, 2003.View at: Publisher Site | Google Scholar
M. D. Lezak, Neuropsychological Assessment, Oxford University Press, New York, NY, USA, 1995.
M. F. Folstein, S. E. Folstein, and P. R. McHugh, “‘Mini mental state’. A practical method for grading the cognitive state of patients for the clinician,” Journal of Psychiatric Research, vol. 12, no. 3, pp. 189–198, 1975.View at: Publisher Site | Google Scholar
J. C. Morris, A. Heyman, R. C. Mohs et al., “The Consortium to Establish a Registry for Alzheimer's Disease (CERAD). Part I. Clinical and neuropsychological assessment of Alzheimer's disease,” Neurology, vol. 39, no. 9, pp. 1159–1165, 1989.View at: Google Scholar
D. C. Delis, J. H. Kramer, E. Kaplan, and B. A. Ober, California Verbal Learning Test (CVLT-II) Manual, Harcourt Assessment Company, San Antonio, Tex, USA, 2000.
D. Wechsler, Manual for the Wechsler Adult Intelligence Scale, The Psychological Corporation, San Antonio, Tex, USA, 3rd edition, 1979.
P. Eslinger, The Iowa Screening Battery for Mental Decline, University of Iowa College of Medicine, Ames, Iowa, USA, 1984.
R. Reitan, “Validity of the Trail Making test as an indicator of organic brain damage,” Perceptual & Motor Skills, vol. 8, pp. 271–276, 1958.View at: Google Scholar
H. Klove and F. M. Forster, “Clinical neuropsychology,” The Medical Clinics of North America, vol. 47, pp. 1647–1658, 1963.View at: Google Scholar
A. T. Beck, R. A. Steer, and G. K. Brown, BDI-II Manual, The Psychological Corporation, Fort Worth, Tex, USA, 1996.
M. Niezel, D. Bernstein, and R. Russel, “Assessment of anxiety and fear,” in Behavioral Assessment: A Practical Handbook, A. S. Bellack and M. Hersen, Eds., Pergamon Press, Oxford, UK, 1998.View at: Google Scholar
S. W. Provencher, “Estimation of metabolite concentrations from localized in vivo proton NMR spectra,” Magnetic Resonance in Medicine, vol. 30, no. 6, pp. 672–679, 1993.View at: Publisher Site | Google Scholar
P. A. Narayana, “Magnetic resonance spectroscopy in the monitoring of multiple sclerosis,” Journal of Neuroimaging, vol. 15, no. 4, supplement, pp. 46S–57S, 2005.View at: Publisher Site | Google Scholar
W. A. Kukull, R. Higdon, J. D. Bowen et al., “Dementia and Alzheimer disease incidence: a prospective cohort study,” Archives of Neurology, vol. 59, no. 11, pp. 1737–1746, 2002.View at: Publisher Site | Google Scholar
L. Letenneur, V. Gilleron, D. Commenges, C. Helmer, J. M. Orgogozo, and J. F. Dartigues, “Are sex and educational level independent predictors of dementia and Alzheimer's disease? Incidence data from the PAQUID project,” Journal of Neurology Neurosurgery and Psychiatry, vol. 66, no. 2, pp. 177–183, 1999.View at: Google Scholar
M. J. Engelhart, M. I. Geerlings, J. Meijer et al., “Inflammatory protemI in plasma and the risk of dementia—the Rotterdam Study,” Archives of Neurology, vol. 61, no. 5, pp. 668–672, 2004.View at: Publisher Site | Google Scholar
P. L. McGeer and E. G. McGeer, “Innate immunity, local inflammation, and degenerative disease,” Science of Aging Knowledge Environment, vol. 2002, no. 29, article re3, 2002.View at: Google Scholar
H. Wersching, T. Duning, H. Lohmann et al., “Serum C-reactive protein is linked to cerebral microstructural integrity and cognitive function,” Neurology, vol. 74, no. 13, pp. 1022–1029, 2010.View at: Publisher Site | Google Scholar
M. B. Pepys and G. M. Hirschfield, “C-reactive protein: a critical update,” The Journal of Clinical Investigation, vol. 111, no. 12, pp. 1805–1812, 2003.View at: Publisher Site | Google Scholar
J. Gussekloo, M. C. L. Schaap, M. Frölich, G. J. Blauw, and R. G. J. Westendorp, “C-reactive protein is a strong but nonspecific risk factor of fatal stroke in elderly persons,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 20, no. 4, pp. 1047–1051, 2000.View at: Google Scholar
V. Pasceri, J. T. Willerson, and E. T. H. Yeh, “Direct proinflammatory effect of C-reactive protein on human endothelial cells,” Circulation, vol. 102, no. 18, pp. 2165–2168, 2000.View at: Google Scholar
P. M. Ridker, C. H. Hennekens, J. E. Buring, and N. Rifai, “C-reactive protein and other markers of inflammation in the prediction of cardiovascular disease in women,” The New England Journal of Medicine, vol. 342, no. 12, pp. 836–843, 2000.View at: Publisher Site | Google Scholar
M. Saito, T. Ishimitsu, J. Minami, H. Ono, M. Ohrui, and H. Matsuoka, “Relations of plasma high-sensitivity C-reactive protein to traditional cardiovascular risk factors,” Atherosclerosis, vol. 167, no. 1, pp. 73–79, 2003.View at: Publisher Site | Google Scholar
J. Gunstad, L. Bausserman, R. H. Paul et al., “C-reactive protein, but not homocysteine, is related to cognitive dysfunction in older adults with cardiovascular disease,” Journal of Clinical Neuroscience, vol. 13, no. 5, pp. 540–546, 2006.View at: Publisher Site | Google Scholar
N. Raz, K. M. Rodrigue, K. M. Kennedy, and J. D. Acker, “Vascular health and longitudinal changes in brain and cognition in middle-aged and older adults,” Neuropsychology, vol. 21, no. 2, pp. 149–157, 2007.View at: Publisher Site | Google Scholar
G. Stebbins, M. Carrillo, M. Moseley et al., “Microstructural integrity of normal-appearing white matter in ParkmIon’s disease: a diffusion tensor imaging study with behavioral correlates,” Neurology, vol. 58, article A200, 2002.View at: Google Scholar
T. Yoshiura, F. Mihara, K. Ogomori, A. Tanaka, K. Kaneko, and K. Masuda, “Diffusion tensor in posterior cingulate gyrus: correlation with cognitive decline in Alzheimer's disease,” NeuroReport, vol. 13, no. 17, pp. 2299–2302, 2002.View at: Publisher Site | Google Scholar
M. Filippi and M. A. Rocca, “MRI aspects of the “inflammatory phase” of multiple sclerosis,” Neurological Sciences, vol. 24, no. 5, pp. S275–S278, 2003.View at: Publisher Site | Google Scholar
S. T. Engeiter, J. M. Provenzale, J. R. Petrella, D. M. Delong, and J. R. MacFall, “The effect of aging on the apparent diffusion coefficient of normal-appearing white matter,” American Journal of Roentgenology, vol. 175, no. 2, pp. 425–430, 2000.View at: Google Scholar
J. A. Kim, M. Montagnani, K. K. Kwang, and M. J. Quon, “Reciprocal relationships between insulin resistance and endothelial dysfunction: molecular and pathophysiological mechanisms,” Circulation, vol. 113, no. 15, pp. 1888–1904, 2006.View at: Publisher Site | Google Scholar
S. Lavi, D. Gaitini, V. Milloul, and G. Jacob, “Impaired cerebral CO2 vasoreactivity: association with endothelial dysfunction,” American Journal of Physiology, vol. 291, no. 4, pp. H1856–H1861, 2006.View at: Publisher Site | Google Scholar
K. M. Flegal, M. D. Carroll, C. L. Ogden, and C. L. Johnson, “Prevalence and trends in obesity among US adults, 1999-2000,” JAMA, vol. 288, no. 14, pp. 1723–1727, 2002.View at: Google Scholar
M. Visser, L. M. Bouter, G. M. McQuillan, M. H. Wener, and T. B. Harris, “Elevated C-reactive protein levels in overweight and obese adults,” JAMA, vol. 282, no. 22, pp. 2131–2135, 1999.View at: Publisher Site | Google Scholar
A. E. Caballero, “Endothelial dysfunction in obesity and insulin resistance: a road to diabetes and heart disease,” Obesity Research, vol. 11, no. 11, pp. 1278–1289, 2003.View at: Google Scholar
C. R. W. Kuhlmann, L. Librizzi, D. Closhen et al., “Mechanisms of C-reactive protein-induced blood-brain barrier disruption,” Stroke, vol. 40, no. 4, pp. 1458–1466, 2009.View at: Publisher Site | Google Scholar
N. J. Abbott, “Astrocyte-endothelial interactions and blood-brain barrier permeability,” Journal of Anatomy, vol. 200, no. 5, pp. 523–534, 2002.View at: Google Scholar
T. K. Shonk, R. A. Moats, P. Gifford et al., “Probable Alzheimer disease: diagnosis with proton MR spectroscopy,” Radiology, vol. 195, no. 1, pp. 65–72, 1995.View at: Google Scholar
W. E. Klunk, C. Xu, K. Panchalingam, R. J. McClure, and J. W. Pettegrew, “Quantitative 1H and 31 MRS of PCA extracts of postmortem Alzheimer's disease brain,” Neurobiology of Aging, vol. 17, no. 3, pp. 349–357, 1996.View at: Publisher Site | Google Scholar